This week was a very engaging and instructive experience on how patience is a virtue.
On Monday, I ran Wes plates on the mid pre frontal cortex region of the brain. Unlike all other days running Wes plates, I ran four plates on the same samples but for both neuroligin1 and glypican4 to determine protein amounts of both in those Wes plates. After running those plates, I did some work on my study design as well as some housecleaning work.
On Tuesday, I checked the Wes runs that we had ran the day before. The result was unexpected. All of the four runs were all off because there was no peak for our biological control, GAPDH. I was shocked and alarmed at the scenario presenting itself. After managing some sample work as well as some data analysis, I called it a day.
On Wednesday, Caitlin and I ran our optimization plate for the MPFC region to ascertain the flaws in the samples. After this, I worked extensively on my study design and analyzed some data.
On Thursday, I looked at our data and ascertained that the biological control was still not present and therefore we needed to run a total protein assay to map out the protein involved. After prepping that plate, I called it a day.
Till next week, signing off.
Thursday, March 24, 2016
Wednesday, March 16, 2016
Week 5
After a week off for spring break, I felt ready to get back to work on my project and maybe gather a lot of useful data for my project as well.
But this week was again not only unique but also a very informative experience.
On Monday, I finished the two Wes plates we needed to collect all the data for the S1BF region of the mice brain. For the plates themselves, I ran two plates testing for Glypican 4 in S1BF samples from day1-7 post injury to complete that region.
During that day as well, I had a meeting with Dr. Theresa Thomas and my senior advisor, Sarah. There, we discussed the challenges facing students who pursue the medical profession and the characteristics that are needed to truly elevate yourself from the pack. All in all, the meeting was very informative and an unique learning experience.
On Tuesday, I started running Wes plates for the MPFC (mid pre frontal cortex) region of the mice brain. After that, I gathered samples from the MPFC region that need to be used for future plates and put them into one box for safe storage in the freezer. I then called it a day after that.
On Wednesday, my senior advisor, Sarah, advised me to work with the Compass software that I use to interpret the Wes results and learn more about the units and values I measure in my experiments. After a few hours tinkering with Compass, I aided our lab manager, Bret, in his study on cage dividers and their effect on mice aggression.
I hope that tomorrow's lab meeting will as informative and unique as this week has been.
Till next week, signing off.
But this week was again not only unique but also a very informative experience.
On Monday, I finished the two Wes plates we needed to collect all the data for the S1BF region of the mice brain. For the plates themselves, I ran two plates testing for Glypican 4 in S1BF samples from day1-7 post injury to complete that region.
During that day as well, I had a meeting with Dr. Theresa Thomas and my senior advisor, Sarah. There, we discussed the challenges facing students who pursue the medical profession and the characteristics that are needed to truly elevate yourself from the pack. All in all, the meeting was very informative and an unique learning experience.
On Tuesday, I started running Wes plates for the MPFC (mid pre frontal cortex) region of the mice brain. After that, I gathered samples from the MPFC region that need to be used for future plates and put them into one box for safe storage in the freezer. I then called it a day after that.
On Wednesday, my senior advisor, Sarah, advised me to work with the Compass software that I use to interpret the Wes results and learn more about the units and values I measure in my experiments. After a few hours tinkering with Compass, I aided our lab manager, Bret, in his study on cage dividers and their effect on mice aggression.
I hope that tomorrow's lab meeting will as informative and unique as this week has been.
Till next week, signing off.
Thursday, March 3, 2016
Week 4
This is an incomplete blog entry as I spent two days in Kansas City for a medical school interview.
But I will give as much information as I can over events that occurred on Monday and Thursday.
On Monday, I ran some more Wes plates with my secondary adviser Caitlin. I ran plates for samples day 14 to day 56 from the S1BF region in mice brains. My results indicate that the run was completed successfully as compared to the disastrous run of the same plate a few days ago. After I completed the Wes plates, I took a break for lunch and organized my notebook and my materials. After that, I called it a day and I prepared for my medical school interview.
Tuesday and Wednesday can be summarized in four words: the interview was difficult. And that is all I am going to say.
On Thursday, after a red eye flight and arriving in Phoenix at 8 in the morning, I caught the light rail to the Arizona Biomedical Collaborative. At the weekly lab meeting, I learned again what not to do in lab studies along with learning some new facets of the medical industry. Overall, I had a very light work day, and after doing some organizing and setting a wes plate for my senior advisor, Sarah, to run, I called it a day.
I hope that on Friday, I can do some more organizing as well as helping others in the lab.
Till next week. Signing off.
But I will give as much information as I can over events that occurred on Monday and Thursday.
On Monday, I ran some more Wes plates with my secondary adviser Caitlin. I ran plates for samples day 14 to day 56 from the S1BF region in mice brains. My results indicate that the run was completed successfully as compared to the disastrous run of the same plate a few days ago. After I completed the Wes plates, I took a break for lunch and organized my notebook and my materials. After that, I called it a day and I prepared for my medical school interview.
Tuesday and Wednesday can be summarized in four words: the interview was difficult. And that is all I am going to say.
On Thursday, after a red eye flight and arriving in Phoenix at 8 in the morning, I caught the light rail to the Arizona Biomedical Collaborative. At the weekly lab meeting, I learned again what not to do in lab studies along with learning some new facets of the medical industry. Overall, I had a very light work day, and after doing some organizing and setting a wes plate for my senior advisor, Sarah, to run, I called it a day.
I hope that on Friday, I can do some more organizing as well as helping others in the lab.
Till next week. Signing off.
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