My
first week at the Arizona Biomedical Collaborative at the Translational
Neurotrauma Lab can be wholesomely described as an entirely new and alien
experience.
Before
joining the research efforts at the ABC, I had a very preconceived notion about
the nature of research. I thought that the professor controls everything within
the lab and dictates every responsibility to high school interns out of a sense
of untrustworthiness. Also, I thought that to pursue research was to throw yourself
into the fire immediately with some training and you proceeded under the
watchful eye of the professors and lab interns.
All
of these notions were proven a mix of truths and untruths.
But
anyway, updates on the past week.
On
Monday, I learned all about the materials needed to properly run the Wes
machine and where they were all stored, which is important information to
consider for experiments.
On
Wednesday, I ran a sham plate for the samples from the VPM region of the brain
for Sprague Dawley rats to ascertain which samples would be appropriate to run
with the injured samples.
On
Thursday, I participated in a lab meeting with all the personnel in the lab.
There, we discussed specific projects that other lab personnel were pursuing as
well as addressing problems and solutions to many issues in the lab.
Afterwards, we watched a presentation on a study on the reduction of aggression
in mice after the addition of a cage divider, where it was ascertained that a
cage divider heavily affected aggression levels in mice. Then, lab personnel
offered other avenues to pursue as well as improvements to make on the
experiment. After the lab meeting, my advisor then again extensively reviewed
the steps needed to run the Wes machine again and I wrote the steps in my lab
notebook.
I
hope that on Friday, I can finally run Wes plates on our own and be well on my
way to maximizing our data.
So
that’s the update for the week.
Till
next week, see you all later.
Of the 2 main chemicals you are testing, are you looking at how to change their rate of production due to TBI or to find out what each does after a TBI?
ReplyDeleteIt's more looking at the presence of these factors in brain samples from different memory related areas of the brain in rats and its role in synaptogenesis.
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