Thankfully, I decided to move back to doing Wes plates this week, as my supervisor Sarah deemed it necessary.
On Monday, we both worked on a Wes plate centering on the antibodies TSP-1 and a mulitplex of TSP-1 and rabbit action or mouse actin. We went through all the procedures as carefully as we could. But in the end, the procedure did not work too well, as the expression of TSP-1 in the samples from the S2BF( barrel cortex) region of the rat brain.
On Tuesday, we then ran a Wes plate comparing the S1BF and S2BF samples from the same animals to see if there was an issue with the tissue along with checking the multiplexing issues. After we completed the procedure, I worked on data entry and called it a day afterward.
On Wednesday, we ran a similar plate as the one on Tuesday, again checking the mulitplexing issues. After that procedure, I labeled tubes to be used for tissue collection and afterward I called it a day.
On Thursday, at the lab meeting, we discussed a lab article on Glutamate amount in relation to a drug, MS-153.
Till next week, signing off.
No comments:
Post a Comment